Monday 02/20/12

9:45 A — 10:00 P

  • Transfer embryos into 30% resin
  • Group meeting / BPS practice talks
  • Transfer embryos into 50% resin
  • New collection cages: 4x snail, yw, and MP09s.
  • embryos into 70% resin.  3 hrs, then 100% resin.
  • images uploaded and sent to Cell
  • Started new protein stains (H3 double / quadriple controls, Rad21 test). (see records)
  • clean and coat large coverslips — do not balance in small ceramic holders without assistance (with KE).
  • Developed wire support for subbing large coverslips.
  • PcG hox/en samples in 70C oven, 9P.
  • Set up ~15 single fly rescue crosses for MP12 rescues.
  • Trying to align more previous STORM data to 3D.
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