9:40 A – 12:15 A
- Flip embryo cages, 9:45A
- Flip collection cages: 3:30P
- Fix embryos: 4:30 P
- Confocal images of latest stains: PcGs with histones and en or hox complex: hot wash was too long (4.5hrs), most in situ signal is lost in all embryos. (except hox Psc).
- PcG proteins and H3 look good in all samples.
- S7 not dissolved (2nd try, 2nd slide lost). Try imaging dissolved clean slide directly prior to mounting using STORM 2).
- STORM2 not recognizing drives through eSATA. saved to disk:D
- S6 500 nm sections most intervening sections etched away in 1.2 hours, but tissue still looks plasticized under high laser (647, 488, not 750).
- Thick sections image much better.