Tuesday 02/21/2012

9:40 A – 12:15 A

  • Flip embryo cages, 9:45A
  • Flip collection cages: 3:30P
  • Fix embryos: 4:30 P
  • Confocal images of latest stains: PcGs with histones and en or hox complex:  hot wash was too long (4.5hrs), most in situ signal is lost in all embryos.  (except hox Psc).
  • PcG proteins and H3 look good in all samples.
  • S7 not dissolved (2nd try, 2nd slide lost).  Try imaging dissolved clean slide directly prior to mounting using STORM 2).
  • STORM2 not recognizing drives through eSATA.  saved to disk:D
  • S6 500 nm sections most intervening sections etched away in 1.2 hours, but tissue still looks plasticized under high laser (647, 488, not 750).
  • Thick sections image much better.
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