Saturday 02/19/2012

10:00A – 5:00P

  • washing out final antibody from in situs.
  • embryos moved to ethanol
  • WGA staining Hox in situs 1:10
  • set up to try cutting thicker samples.  Using Yari’s new walled area for microtome.
  • select Images for Cell feature on Development
  • check images on confocal: good PcG bodies in Pc and Psc (others not checked).
  • engrailed/inv staining not look good in bio, E(Pc)  and tou expression is erratic in older embryos.  No early germband extension embryos in ~4-7 hr collection.
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