11:00 A – 7:30 P, 9:30P – 11:58 P
Discussion
- recommend using homology-fused single labeled probes to test the double labeling efficiency for Hao’s experiment.
- reference call for Bryon
Genomics
- cleaned up Genomics functions
- maxInterval.m
- maxIntervalBed.m
- added new function
- IntervalFuseGaps.m — fuses gaps below a certain size in interval data. Also filters fraction of gaps
- New script (Select_chromatin_regions_032113.m),
- uses IntervalFuseGaps to get gaps of a certain length cut off.
- Loads HMM noise data models for candidate regions and checks color
I think I fixed this bug by doing analysis per chromosome.
Fly Work
- Flip fly stocks
Cell culture work
- restart Kc cells from frozen stocks
- incubator went up to 37 from 28C during 30 min recovery. Hopefully didn’t kill too many cells
- Soval is out of commission. Pelleted cells in mini-centrifuge in sterile cryo-tubes.