Monday 04/07/14

9:30a – 11:30p

Meetings

• lab meeting (see protected notes)
• my journal club presentation (see google-docs presentation)
• initial planning meeting with Steven — need joint write-up, goal to write by Wed

Writing

• finalizing write-up 1 for XZ with Jeff.
• working on rewriting write-up 2 with team.

Chromatin imaging

• samples incubated an extra 24 hours.
• no staining! (in either 647 or 750)
• hypotheses:
  1. maybe the humidity chamber condensation which hydrates the rubber cement dilutes away the probe?
  • try dry hybe comparison tonight
    1. maybe something is really wrong with new hybe dilution mix
  • I think this mix worked sorta the first time the 750 failed last week.
• took bead images (currently on STORM2 hard-drive, need to copy over).
• imaging sample E02

New Staining

• F07-P1 F06-P3 4:4. .33 A647 and .66 A750
• new E04-P1 2.5 uL to .33uL A647
• new E05-P1 1.5 uL to .33uL A647
• use old hybe mix (not hybe dilution mix)
• stain in box without water (see how this goes)

Fly Work

• flipped fly stocks
• balancer Esc almost dead
• Esc/CyO dead, but Esc/Gla still going strong (if stable?)