Category Archives: Chromatin

Protected: Data analysis 10/09/12

Posted on October 9, 2012 by admin

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oligo PAINT in embryos

Posted on September 13, 2012 by admin

DNA FISH / Oligo-PAINT in embryos.  Progress. Note the substantial variation in size.  some of the spots resolve into multiple dense clusters and others are pretty diffuse over a large area. This centromeric AATAT rich repeat covers ~250kb region. Since … Continue reading →

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First pass at H3K27me3 imaging at the ANT-C locus in embryos

Posted on September 9, 2012 by admin
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Tuesday 08/28/12

Posted on August 28, 2012 by admin

9:30 A – 7:00PM, 7:45P – 12:00A 20 min etch Pc-647 sample.  Turnkey inspection looks over-etched, most embryos quite sparse. Forgot that previous MP07 samples have Draq5 not DAPI.   Extracting nuclear masks manually through inverting DAPI channel and using … Continue reading →

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Reflections on Petruk et al Cell 2012

Posted on August 26, 2012 by admin

TrxG and PcG Proteins but Not Methylated Histones Remain Associated with DNA through Replication, Cell (2012), Result 1 (Fig 1):  ‘K27’ ‘K4’ don’t associate with PCNA labeled nuclei.   comment: At gastrulation K27 and K4 levels are much much lower than … Continue reading →

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Protected: H3K27 in vivo structural dynamics

Posted on August 21, 2012 by admin

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annotation for 2012-08-16 AbdA embryos

Posted on August 21, 2012 by admin

imreg_0 Germband extension emb 1 imreg_1 Germband extension emb 1 imreg_2 hr 20+ emb 1 imreg_3  hr 14+  emb 1 imreg_4  hr 14+  emb 1 imreg_5 Germband retraction emb 1 imreg_12  hr 14+  emb 1 imreg_13 partial emb 4-10 hr.  emb … Continue reading →

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Protected: ANT-C locus K27me3 by STORM try 1

Posted on August 15, 2012 by admin

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Protected: tissue specific Methylation state of the BX-C complex by STORM

Posted on August 15, 2012 by admin

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last two weeks in images

Posted on July 5, 2012 by admin
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