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Category Archives: Chromatin
Protected: PcGs in later mitotic stages
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Compare 2D and 3D spot finding results
2D in green, 3D in red (H3-647). Z images are collapsed down into single plane. It looks like brightness differences are substantial, better contrast total photons from 2D imaging (might have expected reverse, 3D apparently doesn’t compensate for photon … Continue reading
Posted in Chromatin, Microscopy
Tagged image analysis, images, STORM
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Protected: PcG in mitosis summary
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Protected: H3K4me3 by confocal and STORM
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chromatin regulation
Selected ubiquitous genes flanking Antp complex: Taf1 (left) and alphaTub84B (right). Need to clone and make probes for these at some point. Look at maintenance of gene activation –how to keep a gene on. Does it require enhancer looping. How … Continue reading
Posted in Chromatin, Research Planning
Tagged Antp, chromatin, H3K27
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testing new antibodies
New antibodies to test from Bernstein lab / Manching EED. Mouse millipore 17-663. .7u/mSUZ12. NG1791537 SUZ12 mouse millipore 17-661 .7. NG1806860 Bmi1 mouse invitrogen 37-5400. .5. 786601A EZH2 39901. Active motif rabbit. 27210001 Mouse anti-H3K27me3. Ab6002. GR50184-2 rabbit anti-H3K27ac active … Continue reading
Posted in Chromatin
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