September 2025 M T W T F S S « Aug 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Categories
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- probe and plasmid building (58)
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- snail patterning (40)
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- Summaries (1,412)
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analysis cell culture cell labeling chromatin cloning coding communication confocal data analysis embryo collection embryo labeling figures fly work genomics hb image analysis image processing images in situs Library2 literature making antibodies matlab-storm meetings modeling MP12 mRNA counting Ph planning presentation probe making project 2 project2 result results sectioning section staining shadow enhancers sna snail staining STORM STORM analysis troubleshooting writing-
GitHub Projects
Protected: Damon Runyon retreat day 2
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Protected: Damon Runyon retreat, Day 1 talks
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Saturday 09/27/14
10:00 am – ?.
project 2
- discussing new analysis methods with Bogdan see post
Data backups
- robocopy — useful built in windows copy function for command line: http://technet.microsoft.com/en-us/library/cc733145.aspx
- Copying files less than 100 MB from (should include all mlists and conventional images), from RAID2 to 4TB fantom drive ‘AlistairBackup’ using new matlab wrapper of robocopy.
- fantom drive AlistairBackup doesn’t want to display its name in Finder (though other properties still record it). Also the original Backup folder I made on this drive is invisible and I can’t make it visible. (Finder reports “F:\ is empty”, even though “F:\Backup\” is not empty and I can browse stuff. Stupid Windows. And I think this is the Hitachi drive that gave me trouble before.
- Zipping Research folder, copying to GRAID
- Set windows automatic backup, nightly at 3 AM, operates on Research folder (and music and pictures).
- configured new RAID drive. May want to reformat as a project2 data-drive, we’ll see who needs these 22TB it first.
- copying Confocal data to GRAID. Let’s delete this from D drive when done so there’s more space on D for nightly backups.
Reorganizing drives
- changed shelves. Moved all 4 8-bay RAID stations to shelf above monitors
- sorted all cables. Added an additional power strip. Now all 7 of my RAID/data arrays can talk to the computer at once.
- cleaned and sterilized desk and keyboard
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Friday 09/26/14
12:15 pm – 12:45 am
Presentation prep
- (rehearsing presentation, 10a-12p)
- put in real DAMID data for epignetic borders.
- wrote script to smooth and normalize chromatin profiles
- revising presentation based on feedback
- 95% confidence bounds are too bright, not enough contrast
Project 2
- annotating data sets (see post)
- sent annotations to Jeff
Chromatin data analysis
- fit chromatic bead data for recent multi-color data sets
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Protected: project 2, annotating data
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Thursday 09/25/14
8:15 am – 7:00 pm,
remotely 10:00 pm – 12:00 am
Project 2
- double-checking and documenting results from yesterday. (see posts)
- planning discussion with Jeff (see email)
Presentation Prep
- Rewriting STORM simulation from input data
- working on slide layout
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Protected: project 2, progress update, 09/25/14
Posted in Genomics
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Wednesday 09/24/14
9:30 am – 12:45 am
project 2
- working on data analysis, see post.
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Protected: project 2 notes: 09/24/14
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Protected: project 2: meeting notes 09/24/14
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