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Tag Archives: sna
Friday 07/01/11
10:00 A – 6:00P Analyze sna data MP06 Hz at cc13-14 transition, substantially less y. Seems to be a temporal transition. Analyze G4B LacZ data (see p0st)
Thursday 06/30/11
9:30 A – 4:10 P Data analysis: focus on sna rescues. Imaging: sna 2.8 enhancer rescues. Partially cooled lasers/lens lead to drift during image acquisition. Stepsize is substantially greater than 330 nm and mRNA are not detected in consecutive layers … Continue reading
Protected: Issues with detection sensitivity
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Posted in Microscopy, Shadow Enhancers
Tagged controls, mRNA counting, sna, y-BAC
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Protected: Exploring loss of sna expression
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Posted in Research Planning, Shadow Enhancers
Tagged chromatin, sna, trouble shooting
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Protected: sna rescue, shadow/distal removed, dramatically lowers expression
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Posted in Shadow Enhancers
Tagged analysis, mRNA counting, rescue, sna
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Protected: Weaker effect on reporter when removing distal sna enhancer
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Posted in Shadow Enhancers
Tagged analysis, mRNA counting, sna
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Protected: High mRNA expression upon proximal sna enhancer deletion
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Posted in Shadow Enhancers
Tagged analysis, mRNA counting, sna
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Tuesday 06/28/11
9:30 A-7:00P, 7:40-8:40, 10:00P- 11:10P Data alignment Flip sna[D] and hb[D] flies into new bottles, keep at 25C. Compare hb data by image type. confirm fairly dynamic expression prevents easy super-position of patterns during cc13. February’s data format doesn’t fit … Continue reading
Monday 06/27/11
10:00 A — 6:45 P, 7:30P – 9:20 P Discussion with Mounia and Jacques from Gordon Conference Discussion with ML from Gordon Conference observations. Set up priorities. Progress Report. Errors in data analysis for Espl, vnd double null embryo analysis. … Continue reading
Protected: snail y Hz quantification
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Posted in Shadow Enhancers
Tagged controls, mRNA counting, shadow enhancers, sna
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