Author Archives: admin

Protected: project 2 update 07/08/14

Posted on July 8, 2014 by admin

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Tuesday 07/08/14

Posted on July 8, 2014 by admin

10:00 am – 8:00 pm, 11:00 pm – 11:50 pm Sequential staining F06 at least stains this time, some spots quite clear. However staining not very good and STORM pretty weak. Tried flowing more secondary for an extra 2 hours … Continue reading →

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Monday 07/07/14

Posted on July 7, 2014 by admin

9:00 am – 5:00 pm, 6:00 pm – 11:00 pm Meetings project 2, team meeting, see email lab meeting, see notes journal club, see notes Sequential staining imaging BX-C. Looks bright and clear again. try competing out S3 probe with … Continue reading →

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Journal club: whole animal light-field microscopy

Posted on July 7, 2014 by admin

(Prevedel et al Nature Methods 2014) Light-field microscopy – capture 3D strcuture of object in single snapshot first developed in 2006 array of micro-lenses, different parts of sensor get different z-fields going deep spread out image, lower signal to noise, … Continue reading →

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Protected: Group meeting 07/07/14

Posted on July 7, 2014 by admin

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Sunday, 07/06/14

Posted on July 6, 2014 by admin

10:15 am – 4:00 pm, 10:30 pm – 12:00 am Imaging today single color finished imaging Do8toD12 started data transfer started imaging C02. Too many spots per cell in many cells. This is not good, something weird with this locus … Continue reading →

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Saturday 07/05/14

Posted on July 5, 2014 by admin

10:30 am – 9:00 pm STORM imaging today Sequential hybes repeat staining of F06 not much improved on BXC-F06 try 2. Hardly see any stain (just background?) Attempting to image these anyway. Imaging new stain: D08toD12 stains look great. Switching … Continue reading →

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Friday, July 4th, 2014

Posted on July 4, 2014 by admin

10:00 am – 1:10 am Discussion sent feedback to Ting about proposed revisions for fig2 sent comments to Hao about presentation for this evening project 2 team meeting meeting with XZ discuss project 2 partitioning (see emails). Analyzing STORM data … Continue reading →

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Thusday 07/03/14

Posted on July 3, 2014 by admin

9:50 am – 7:30 pm coding found and fixed bug in ClusterStats.m Sequential hybes blead-back in of probe signal: probably a sign of not sufficiently sealed chamber. Try air drying sample and then sealing really tight. hybe2 stain initially very … Continue reading →

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Wednesday 07/02/14

Posted on July 2, 2014 by admin

10:00 am – 11:35 pm Goals Remove panel (c), add multiple examples of 405-cy5 BX-C spots for oligo-secondaries for previous panel (e) Fix feducialDriftCorrection — github still preserves the lowercase name. maybe best fix is to correct spelling (FiducialDriftCorrection) write … Continue reading →

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