10:15 am – 4:00 pm, 10:30 pm – 12:00 am
Imaging today
single color
- finished imaging Do8toD12
- started data transfer
- started imaging C02. Too many spots per cell in many cells. This is not good, something weird with this locus (or probeset), might be best to skip it for now.
- took a conv mosaic and some STORM images to document these observations in C02.
- set up new scan of E05
Sequential stain troubleshooting
- went to set up new sequential stain, found previous probes F03 to F05 p3, and F06-p3 not P1.
- I suspect this is the reason I could not get P1 to stain
- also means can’t trust the BX-C imaging data from the last few days. Too bad, have to repeat all that.
- set up new stains
- F03 to F05-p3, .75 uL each + S3 1.3 uL, + F06-p1, 1.5 uL
Oligo secondaries
- finish writing methods section for simulations
- sent this and some minor tweaks / revisions to Ting et al.
Project 2 probes
- set up T7 reactions
- started reactions without RNasin. Added at 20min.