Author Archives: admin

Sunday 05/11/14

Posted on May 11, 2014 by admin

12:00p – 5:00p Finish probe making: E05-E12, F01-F08 p3 Run gel Run clean up. New stains F02-p3, F04-p1 F02-p3, F03-p1 F03-p3, F04-p1 New Probes to make L3: B02 – B09 L2: F03, F04, F05, F06, F07. L3: F09, F10, F11 … Continue reading →

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Monday 05/19/14

Posted on May 10, 2014 by admin

9:00a – 6:00p My lab meeting feedback Complement area analysis as Radius of gyration of molecule positions. volume analysis for some loci. log transform spot densities to avoid saturation. Area comparison plots normalized to largest spot fraction of single vs. … Continue reading →

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Friday 05/09/14

Posted on May 9, 2014 by admin

Goals Project 2 relaunch batch analysis troubleshoot output send new writeup to Xiaowei STORM keep rolling. Samples to Image L2G06-p1-A647 L2G08-p1-A647 Samples to stain F01 – F06 P3 Probes to finish F01-F08 and E05 – E12 F04(EPc) + F02(En) vs … Continue reading →

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Tuesday 05/06/14

Posted on May 6, 2014 by admin

9:00a – 12:00p Conference Prep Review speaker list, read up on papers. Travel to Janelia. Project 2 see notes

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Protected: project 2: update 05/06/14

Posted on May 6, 2014 by admin

There is no excerpt because this is a protected post.

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Monday 05/05/14

Posted on May 5, 2014 by admin

9:00a – 5:00p Lab Meeting Guest speaker, see post. Multicolor STORM more trouble-shooting observed that 647 images look particularly weak, especially given the brightness of these spots. 15 min of max 405 completely bleaches the 647 spot. (~50K frames). 561 … Continue reading →

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lab meeting 05/05/14

Posted on May 5, 2014 by admin

Jean-Ju Chung, Spatial Organization of CatSper in Sperm (former collaborator of Sang-He) Clapman lab results now published, to appear soon. background ejaculated sperm not motile outside female environment. Hence ‘not fertile’. what’s the difference? Bicarbonate rich, Albumin. pH changes sharply … Continue reading →

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Sunday 05/04/14

Posted on May 4, 2014 by admin

10:30a – 10:00p Goals Poster revisions as detailed by XZ. Relaunch Oligo-Array analysis — matlab control script killed by Odyssey. Test new 2-clr STORM stains Questions for RC computing use general queue / infiband to analyze data on Odyssey CPUs … Continue reading →

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Protected: Project 2 update: 05/04/14

Posted on May 4, 2014 by admin

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Saturday 05/03/14

Posted on May 3, 2014 by admin

6:30p – 8:30p STORM setup imaging of E04 high background. Otherwise dyes look very bright, switching looks great.

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