Friday 05/09/14

Goals

• Project 2
  • relaunch batch analysis
  • troubleshoot output
  • send new writeup to Xiaowei
• STORM
  • keep rolling.

Samples to Image

1. L2G06-p1-A647
2. L2G08-p1-A647

Samples to stain

• F01 – F06

P3 Probes to finish

• F01-F08 and E05 – E12
• F04(EPc) + F02(En) vs F09 (En TAD)
• Per sample
  • 3.5 uL 100 mM primer
  • 4 uL 5x RT buffer
  • 2 uL dNTPs (10 mM)
  • 1 uL RNAsin inhibitor
  • .2 uL Maxima
  • 10 uL RNA
• master mix 18x
  • 63 uL primer
  • 72 uL RT buffer
  • 18 uL RNAsin inhibitor
  • 36 uL dNTPs
  • 4 uL Maxima

New Probes to make

• L3: B02 – B09
• L2: F03, F04, F05, F06, F07. L3: F09, F10, F11 (all 2 color),