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Tuesday 09/22/15
9:00 am Job Apps working on figures for Research Statement (11a – 2p, 8p-10p) some progress on corresponding text. Manuscript tweaking alternative version of EDF 3. (9:30 – 10:45am) revising response to reviewer letter 2pm – 4:30 pm, 6:00pm – … Continue reading
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Monday 09/21/15
9:00 am – 7:00 pm, 9:00 pm – 10:00 pm Manuscript revisions revising main text in response to XZ comments figure adjustments in response to XZ comments discussed main text revisions simplified Ph KD discussion simplified PRE discussion simplified fixation … Continue reading
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Sunday 09/20/15
10:30 am – 7:00 pm Job apps writing research statement Figure revisions for chromatin see XZ notes Experiments DNA digestion following IVT (dilute 2x add DNase I from NEB IVT quick kit) RNA purification quantify yield transfect new KC cells. … Continue reading
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Saturday 09/19/15
10:40 am – 1:30 pm New Ph KD set up new Ph KD with last drops of previous dsRNA batch for ph-p and ph-d (well, I still have some ph-d left). Also did Pc KD as a bonus. ran 10% … Continue reading
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Friday 09/18/15
9:05 am – 5:15 pm Chromatin manuscript revising ED fig 3 I think we can make the fig 1 images work might need to do denoising requested by reviewer also tuned up previous examples and improved contrast (still only 1 … Continue reading
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Thursday 09/17/15
9:15 am – 10:00 pm Manuscript revising main text chromatin experiments helping BB do deep-seq prep KapaQuant results: concentrations lower than expected, (only most concentrated of the dilutions look good) suspect a problem with the beads Tapestation quantification — no … Continue reading
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Wednesday 09/16/15
10:00 am job app stuff working on description of institutions with due dates for letter writers Career Advising appointment scheduled for Oct 16th, 10:00 am email cover-letter and CV to in advance kargere@fas.harvard.edu Ph manuscript tweaks to fig 5 discussed … Continue reading
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Protected: CONTI center notes
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Sunday 09/13/15
3:45 pm – 7:45 pm STORM C03 imaging finished ~4:00 pm (17 hours) replaced with fresh buffer, setting up new tile scan. Fixed Kc cells plated on 18mm round coverslips last night. started new imaging round started DaoSTORM running on … Continue reading
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Saturday 09/09/12
12:15 pm – 5:30 pm, 8:40 pm – 11:20 pm Ph manuscript revisions went through and main text revisions, updated and replied went through supp text revisions, updated and replied updated Supp Figs (updated SF1, check with NF about SF11 … Continue reading
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