10:40 am – 1:30 pm
New Ph KD
- set up new Ph KD with last drops of previous dsRNA batch for ph-p and ph-d (well, I still have some ph-d left). Also did Pc KD as a bonus.
- ran 10% short on ph-p. And I’m not sure if this dsRNA is still up to snuff, its been thawed at least 5 times and in the -80 for over a month.
- knockdown started at 12:10 pm.
- started prepping new dsRNA for ph-p and ph-d.
- PCR / cleanup column doesn’t seem to be ideal — I spec’d the DNA (~70ng/uL and 160 ng/uL respectively for ph-p and ph-d) and the 230 peak is as high as the 280.
- set up T7 reactions anyway. Might run a gel after the DNase treatment and RNA cleanup (do tomorrow?)
- should probably do another round of QPCR on these RNA isolations before deep sequencing to check KD given issues.
Job app stuff
- working on revised statement.
- updated CV with CB comments.