Category Archives: General STORM

issues with non-blinking weak spots

Posted on November 15, 2012 by admin

The very bright, round foci clearly do not correspond to objects in the conventional image and are probably all artificats from non-switching foci. These dots start off at moderate single molecule brightness (can’t eliminate them just using higher threshold cutoff … Continue reading →

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Troubleshooting new hard-drive install

Posted on November 8, 2012 by admin

Windows doesn’t like talking to new disk drives, and repeatedly connecting new drives seems to create a problem.  Especially when the drives are formatted for Mac. Symptom: The newly plugged in drive does not appear. In start menu, search for … Continue reading →

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Installing DaoSTORM for windows

Posted on September 14, 2012 by admin

Into the rabbit hole! DaoSTORM requires 64 bit C-libraries, which requires a 64 bit compiler, which to build first requires an installation of a c-32 bit compiler.  Also requires Fortran libraries in Lapack which requires the program CMAKE to install … Continue reading →

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DaoSTORM for Matlab

Posted on September 13, 2012 by admin

  mufit starts writing  bin files immediately (good for memory) First we need a watcher: A good introduction to using WMIC http://isc.sans.edu/diary.html?storyid=2376 (Windows Management Instrumentation Command) [status result] = dos(‘wmic process where name=”python.exe” list brief’); running = strfind(result,’python.exe’); Running head-to-head comparison of … Continue reading →

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First pass at H3K27me3 imaging at the ANT-C locus in embryos

Posted on September 9, 2012 by admin
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