Category Archives: Summaries

6:00 pm – 7:00 pm Embryo staining just going for labeling — set heat block to 100C spec new library: label just new library, 3 uL in 20 uL buffer. control embryo, restain Friday’s failed sample with L4E24 P2/S2

9:00 am – 7:15 pm Meetings lab meeting (see notes) embryo staining & imaging stained BX-C locus with Bit 1 (9:00 am) rinsed out stain broke coverslip 1 after it detached from the plastic petri dish before the the nail … Continue reading →

9:40 am – 5:00 pm Goals & progress prep and stain embryos with new L7 library. -> DONE order food for group meeting. -> DONE. pick up suit for interviews. -> DONE. work on equipment lists. -> Extracted parts list … Continue reading →

11:00 am – 6:30 pm embryo smFISH optimization testing quality of methanol stored embryos from yesterday: 6 mo and 2 year embryo batches stained with my in situ protocol I optimized in the Levine lab 6 mo sample directly labelled … Continue reading →

9:40 am – 7:00 pm Reading Recommendations for junior scientists: https://drmaltman.wordpress.com/2014/09/17/10-simple-steps-to-building-a-reputation-as-a-researcher-in-your-early-career/ I don’t have time to make it to the lecture on this today, but some simple suggestions worth bearing in mind from Micha Altman I should probably update my … Continue reading →

10:10 am (back from UCSD interview) Probe synthesis L7 10kb BXC library — amplifying amplifies and saturates about cycle 24 (ran 27 cycles which was probably extra but should still make good probe) purified amplification, ready for T7 reaction don’t … Continue reading →

9:15 am – 5:00 pm Tasks collect and match primers for DSB library training GN on AO STORM3 Boran helped review protocol: MicAO software (not AO folder) use hal to select record and save frames and then use AO software … Continue reading →

9:30 am – 7:00 pm Our work, “Super-resolution imaging reveals distinct folding for different epigenetic states” is now out in Nature! Our work, “Chromatin topology is coupled to Polycomb Group protein subnuclear organization” is now out in Nature Communications Tasks … Continue reading →