Category Archives: Microscopy

Laser powers: 750: 104 mW 647 : 150 mW 561: 150 mW 488: 150 mW 405: 1.5 mW Sample 2H.  750 switching nicely. 647 very bright conventional images.  Under high laser power image still inverts — low nuc singal high … Continue reading →

Laser Powers: 750: 100 mW 647: 15o mW 561: 150 mW 488: 15o mW sample ‘4H’ (histone H3 stained in all 4 channels as an alignment control). etched sample 22 min.  Looks like all resin is dissolved. very weak switching. … Continue reading →

Laser powers 750: fix to 100 mW 647: 175 mW 561 (at 200 mW activation) fix to 150 mW 488:  200 mW 405: 1.5 mW Imaged S6 500 nm, single section with hox expression dot. Arraying sample of S7, engrailed … Continue reading →

  Laser powers 750: 102 mW 647: 200 mW 561: 150 mW 488: 200 mW 405: 1.5 mW imaging slide S7 750 looks pretty good, Dm01 better in young embryos. Other channels look like resin is still strong in outline. … Continue reading →

STORM imaging 2/16/12 Laser calibration at output: 750 100 mW, 647 200 mW 561 100 mW (200 at laser) 488 200 mW 405 1.3 mW (max why low?) substantial resin still on slide S7 etched yesterday for 30 min. etched … Continue reading →