Category Archives: Microscopy

Sunday 11/06/11

Posted on November 6, 2011 by admin

10A – 11:45A, 2:ooP-4:00P, 6:00P-11:50P Turnkey 60x works alright for mitotic sorting of DAPI staged embryos — constructed large well coverslip with channels for removing excess buffer.  In mininmal buffer embryos don’t float around and are easier to pick off … Continue reading →

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Saturday 11/05/11

Posted on November 5, 2011 by admin

2:30 P — 6:45 P chambered slides work well for checking and tracking single embryos. H3K4 works, PolII has sharp background spots but no obvious nuclear stain. Post-fix embryos and then rinse and then do sorting, prior to ethanol and … Continue reading →

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Protected: Issues with detection sensitivity

Posted on June 29, 2011 by admin

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Protected: MP09 intensity sensitivity

Posted on June 23, 2011 by admin

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mRNA counting control

Posted on June 6, 2011 by admin

  Double labeling, raw image (left), and competition labeling, raw image (right)                         Images are max-projects through the z-dimension.  hatches mark dotfinder located centroids.  Yellow stars mark mRNA transcripts found … Continue reading →

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Protected: new hb analysis data

Posted on April 19, 2011 by admin

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more imaging of bursting

Posted on April 13, 2011 by admin

cadN images:  

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Imaging bursting?

Posted on April 12, 2011 by admin

Imaging transcriptional bursting directly? .  

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to image

Posted on April 9, 2011 by admin

Slides to image still: 04/06/10: C33, C55, untouched 22C and 30C 0-4 hr slides.  Need cc13 embryos 01/07/10: 3x snail in vnd[D38] 01/07/10: E(spl) x vnd[D38], E(spl); E(spl)/HbZ

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Protected: enhancer gradient analysis (hb + sna)

Posted on March 29, 2011 by admin

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