2:30 P — 6:45 P
- chambered slides work well for checking and tracking single embryos.
- H3K4 works, PolII has sharp background spots but no obvious nuclear stain.
- Post-fix embryos and then rinse and then do sorting, prior to ethanol and resin.
- Sorting on Zeiss700, 20x sufficient for detailed stage sorting, 10x works well to select embryos off of coverslips.