Category Archives: Chromatin

Protected: project 2 coding update

Posted on April 19, 2013 by admin

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Protected: Activator oligoPaints: results summary 04/05/13

Posted on April 5, 2013 by admin

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Protected: issues with ph-FLAG round 1

Posted on April 5, 2013 by admin

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Protected: STORM3 fab7 data

Posted on April 4, 2013 by admin

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oligopaint probe making planning

Posted on April 1, 2013 by admin

emPCR ready libraries: AbdA AbdB Ubx en-locus 100kb Black 100kb Yellow 100kb Green 100kb Synthesis schemes: Teritaries + Nicking PCR using extended primers = Forward primer (21bp) + Secondary binding site (32 bp) Nick off R primer Denaturing PAGE Gel … Continue reading →

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Protected: preliminary BX-C embryo

Posted on March 25, 2013 by admin

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Protected: Alternative library and probe designs

Posted on March 10, 2013 by admin

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embryo sections

Posted on March 5, 2013 by admin

some sections lie nice and flat, others get substantial ripples. Is this knife compression? too large a section in x-y? (seems to correlate). Too thick a section? (seems also to be true in my experience).

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Failures update

Posted on March 1, 2013 by admin

Fly Genetics Failed knockdown: 2x Mat alpha tub driving any of the TRiP valium 20 or valium 22 PcG target shRNAs. Gives viable F1 and F2s or infertile F2s. No PcG phenotypes. Kill all current crosses Kill all expanding Gal4 … Continue reading →

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Filtering interval data

Posted on February 27, 2013 by admin

Allowing small gaps. Some very brief changes in chromatin type may not be real: Need to allow gaps – small misclasifications rrrrrrrrrrr kk rrrrrrrrrrrrrrrrr bbb rrrrrrrrrrr should still be scored as a long red region. The kk/bbb might be background, … Continue reading →

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