Monthly Archives: June 2011

Thursday 06/30/11

9:30 A – 4:10 P Data analysis: focus on sna rescues. Imaging: sna 2.8 enhancer rescues. Partially cooled lasers/lens lead to drift during image acquisition.  Stepsize is substantially greater than 330 nm and mRNA are not detected in consecutive layers … Continue reading

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Wednesday 06/29/11

9:40 A –3:30 P, 7:50 P – 9:20 P Finish ON confocal MP07Hz_snaD image 2.8Hz_snaD rescue for counting.  — still drift issue during acquisition as lens heats. Literature review: Set1 not Trx is major H3K4 trimethyltransferase (Lis) Trans-activation in mammalian … Continue reading

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Protected: Issues with detection sensitivity

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Protected: Exploring loss of sna expression

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Protected: sna rescue, shadow/distal removed, dramatically lowers expression

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Protected: Weaker effect on reporter when removing distal sna enhancer

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Protected: High mRNA expression upon proximal sna enhancer deletion

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Tuesday 06/28/11

9:30 A-7:00P, 7:40-8:40, 10:00P- 11:10P Data alignment Flip sna[D] and hb[D] flies into new bottles, keep at 25C. Compare hb data by image type.  confirm fairly dynamic expression prevents easy super-position of patterns during cc13. February’s data format doesn’t fit … Continue reading

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Monday 06/27/11

10:00 A — 6:45 P, 7:30P – 9:20 P Discussion with Mounia and Jacques from Gordon Conference Discussion with ML from Gordon Conference observations.  Set up priorities. Progress Report. Errors in data analysis for Espl, vnd double null embryo analysis. … Continue reading

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Protected: snail y Hz quantification

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