Monthly Archives: May 2011

shared multi-user git repo

Posted on May 31, 2011 by admin

Notes on making a github public repository into a shared project modifiable on multiple computers by multiple users.  (Applied today for my GitHub project AlistairBoettiger/Image_Analysis, added user AlistairMobile. First new users need github account.  Then need git installed on the … Continue reading →

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Tuesday 05/31/11

Posted on May 31, 2011 by admin

8:40 A — 9:15 A, 10:10 A – 7:00P, 8:00P-9:30P, 10:15P-12:00A Finish confocal run, 8 embryos imaged. Start confocal image of Espl null, vnd null (3 images taken today) Fix embryos 1-5 hrs: G4B x vnd, G4B x 40xUAS-C8G, 20xUAS-C8G, … Continue reading →

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Monday 05/30/11

Posted on May 30, 2011 by admin

11:30 A -10:15 P Finish confocal run of 1x bcd (15 embryos imaged). Data organization (see post). Making buffers A1 and A2 for nuclei isolation protocol. single protein pulldown and imaging published (Jain 2011, Nature) attempting nuclei isolation — only … Continue reading →

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Database organization

Posted on May 30, 2011 by admin

Transfer and organize image data All image data moved onto G-RAID backup. Files renamed yyyy-mm-dd.

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Sunday 05/29/11

Posted on May 30, 2011 by admin

1:40 P – 4:00 P, ON confocal run finished at 2:30P, 13 embryos imaged. Set up new imaging scan using 1x bcd embryos.  Hopefully optics stay warm from previous ON, might have drift issues.  17 embryos. Flip collection cages, 3:40P.

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Saturday 05/28/11

Posted on May 28, 2011 by admin

8:40 A – 9:30 A, 10:30A – 2:00P, 7:00P – 8:00P, 8:50P -10:30P Confocal MP01 Collect virgins AM and PM Confocal MP02 New collection cages, gal4-bcd-utr x UAS-vnd. New cage: MP08; snaD/HbZ x snaD/HbZ

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Friday 05/27/11

Posted on May 27, 2011 by admin

10:30 A – 6:00P, 8:00P – 10:20P confocal: competition labeling control, 10:30A – 1:00P. ordering plasmid and primers for mCD8:GFP (modified) from AddGene.  File MTA paperwork for HHMI and reimbursement paperwork for MCB collect virgin sna[D]/HbZ and Gal4-bcd-3UTR give all … Continue reading →

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mCD8-GFP probe

Posted on May 27, 2011 by admin

Order primers hsp70 and SV40 primers  (see primer database), to sequence mCD8:GFP + intron probe.  total length from hsp70 through polyA SV40 is 2.1kb Order AddGene plasmid mCD8::GFP-1xUAS. File MTA with Berkeley Office. File reimbursement request with MCB purchasing.

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Thursday 05/26/11

Posted on May 26, 2011 by admin

9:30 A — 5:00 P, 11:20 – 12:00A continue confocal run.  Heating drift correction seems alright but many embryos have the wrong plane (sometimes too low, sometimes too high).  Need to take more care in specifying upper planes of imaging. … Continue reading →

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Wednesday 05/25/11

Posted on May 25, 2011 by admin

1:50 P-7:00P, 8:15P -11:00P Travel back from Janelia check linearization PCRs New cage Gal4-bcd 3’UTR x UAS-twi-twi New cage Gal4-bcd 3’UTR x LacZ New cage: Gal4-bcd 3’UTR x 40xUAS-GFP-CD8 Confocal MP09 Hz 22C.  16 embryos, 50 slices at .33 um … Continue reading →

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