Daily Archives: May 25, 2011

Wednesday 05/25/11

Posted on May 25, 2011 by admin

1:50 P-7:00P, 8:15P -11:00P Travel back from Janelia check linearization PCRs New cage Gal4-bcd 3’UTR x UAS-twi-twi New cage Gal4-bcd 3’UTR x LacZ New cage: Gal4-bcd 3’UTR x 40xUAS-GFP-CD8 Confocal MP09 Hz 22C.  16 embryos, 50 slices at .33 um … Continue reading →

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probe making: chromatin condensation

Posted on May 25, 2011 by admin

Linearized templates for: inv A, E(Pc) A, tou A, en A (in order). Gel extracted and purified. All concentrations ~20 ng/uL.             Synthesis setup: [everything with T7 :), all templates same conc. ]. 20x master: 100 … Continue reading →

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