Daily Archives: May 30, 2011

11:30 A -10:15 P Finish confocal run of 1x bcd (15 embryos imaged). Data organization (see post). Making buffers A1 and A2 for nuclei isolation protocol. single protein pulldown and imaging published (Jain 2011, Nature) attempting nuclei isolation — only … Continue reading →

1:40 P – 4:00 P, ON confocal run finished at 2:30P, 13 embryos imaged. Set up new imaging scan using 1x bcd embryos.  Hopefully optics stay warm from previous ON, might have drift issues.  17 embryos. Flip collection cages, 3:40P.