11:30 A -10:15 P
- Finish confocal run of 1x bcd (15 embryos imaged).
- Data organization (see post).
- Making buffers A1 and A2 for nuclei isolation protocol.
- single protein pulldown and imaging published (Jain 2011, Nature)
- attempting nuclei isolation — only get lightly or heavily fragmented embryos, but fail to separate out nuclei.
- Fix embryos, 2-4 hrs (gal4-bcd-3’utr x twi-twi, LacZ II, 5xUAS-mCD8-GFP (C8G) 20xC8G, 40xC8G). Leave out Triton-X, use extra washes (trying to eliminate biotin background)
- Confocal MP08, snaD ON, started 9:30P, 10 embryos
- Found one embryo AP half snail on half off? bistable?