Tuesday 05/31/11

8:40 A — 9:15 A, 10:10 A – 7:00P, 8:00P-9:30P, 10:15P-12:00A

  • Finish confocal run, 8 embryos imaged.
  • Start confocal image of Espl null, vnd null (3 images taken today)
  • Fix embryos 1-5 hrs: G4B x vnd, G4B x 40xUAS-C8G, 20xUAS-C8G, 5xUAS-C8G, G4B x LacZ, G4B x UAS-twi-twi,  MP08; sna[D] hets,
  • Flip collection cages again, 6p
  • rewriting imviewer_lsm to: read file info from lsm encoded data, don’t import the whole stack at once, perform projections in a single step.
  • Try out Fiji.  Successfully uploads lsm stacks once memory allocation expanded to 40GB.    Colors images on uint8 scale — uint16 data all looks saturated.
  • Adding multiple users to git repository, see post.
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