Daily Archives: May 27, 2011

Friday 05/27/11

10:30 A – 6:00P, 8:00P – 10:20P confocal: competition labeling control, 10:30A – 1:00P. ordering plasmid and primers for mCD8:GFP (modified) from AddGene.  File MTA paperwork for HHMI and reimbursement paperwork for MCB collect virgin sna[D]/HbZ and Gal4-bcd-3UTR give all … Continue reading

Posted in Summaries | Leave a comment

mCD8-GFP probe

Order primers hsp70 and SV40 primers  (see primer database), to sequence mCD8:GFP + intron probe.  total length from hsp70 through polyA SV40 is 2.1kb Order AddGene plasmid mCD8::GFP-1xUAS. File MTA with Berkeley Office. File reimbursement request with MCB purchasing.

Posted in probe and plasmid building | Comments Off on mCD8-GFP probe