10:30 A – 6:00P, 8:00P – 10:20P
- confocal: competition labeling control, 10:30A – 1:00P.
- ordering plasmid and primers for mCD8:GFP (modified) from AddGene. File MTA paperwork for HHMI and reimbursement paperwork for MCB
- collect virgin sna[D]/HbZ and Gal4-bcd-3UTR
- give all extant 11/CyO; hb[D]/HbZ flies to Mike Perry. Line is trying hard to die out.
- Get MP08 Hz ; sna[D]/CyOZ flies from Perry, separate males, put females into vials to expand.
- Flip collection cages, 11:00A
- Biophysics website meeting 1p – 3:00 P
- break-room cleaning 4p-5p
- Discuss LacZ shadows ms with ML
- Confocal, control image: slide 15 failed, slide 12 double-labeled y-full
- Collect males from mCD8:GFP multi-X UAS lines. Flip bottles.
- Collect virgin sna[D]/CyoZ and Gal4s
- Confocal MP01 Hz 10 embryos, started at 9:40 P.