Monthly Archives: April 2011

Saturday 04/30/11

Posted on April 30, 2011 by admin

11:30 A -7:00P Stop confocal run, all 29 embryos seem to have run correctly.  Copying data. Take plates out of 37C, move to 4C. Mendeley tabling at cog science conference (12:50-2:00P) Confocal 19 embryos C55 30C (warm up 4:00P-5:30P.  Started … Continue reading →

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Friday 04/29/11

Posted on April 29, 2011 by admin

10:00 A — 10:30 P Test gel for yellow exon fragment cloning for competition assay Test probe synthesis reactions.  Results. Dot finding code and data (MP01_c collection) to Jacques. Gel extract yellow exon fragment clone.  High MgCl worked best, cloning … Continue reading →

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probe making

Posted on April 29, 2011 by admin

cloning y fragment 2nd intron: Gel extracted bright band amplified from linear full-length yellow.   test gel for probe synthesis, rather variable efficiency. Probe order: bio-probes (3uL diluted), then dig probes (at 1uL diluted). hb-full, pnr-exon (A), y-full, sna, ths(A) … Continue reading →

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Probe synthesis

Posted on April 28, 2011 by admin

Making probes.  Reaction setup sketch:     Ordered more bio mix and dig mix.  Enzyme stocks still okay. Attempt new y exon fragment 2 synthesis.

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Thursday 04/28/11

Posted on April 28, 2011 by admin

8:40A – 5:00 P, 10:30 P – 12:00A in vitro transcription reactions (see probe making entry) retry PCR of 2nd exon fragment for y: 1.5 2.5 and 5 uL of MgCl2 and standard clone from linearized y-full length transcript. work … Continue reading →

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Wednesday 04/27/11

Posted on April 27, 2011 by admin

1:30 P – 3:00P Arrive back from Boston. Updated goals to ML Arrange travel days for Janelia. Flip hb early rescue vials. Expanding from single-line vials BAC3 early rescues again.

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Wednesday 04/20/11

Posted on April 20, 2011 by admin

10:10 A – 4:00 P, 8:15P – 11:15P Give MP08 sna[D] cages to Perry for collection. Reviewing hb-enhancer noise data Working on Postdoc talk presentation Check and flip bottle flats at 25C Flip bottle stocks at 18C Further revisions to … Continue reading →

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Protected: new hb analysis data

Posted on April 19, 2011 by admin

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Tuesday 04/19/11

Posted on April 19, 2011 by admin

9:30 A – 4:00 P, 7:50P – 11:15 P Getting supplies for Blair’s post-Qual party Flip collection plates 10A. Test gel of PCR amplification of plasmid DNA (using just DNA pricks, no pipetting).  Most look good, proceed to probe synthesis. … Continue reading →

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NSF GRFP annual report

Posted on April 19, 2011 by admin

That time of year again, listing progress.  Never get any feedback on these so I’m getting hasty in my presentation. Achievements listed this year: NSF Activities Report 2010-2011 I have been engaged in mathematical modeling of transcriptional regulation through release … Continue reading →

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