Thursday 04/28/11

8:40A – 5:00 P, 10:30 P – 12:00A

  • in vitro transcription reactions (see probe making entry)
  • retry PCR of 2nd exon fragment for y: 1.5 2.5 and 5 uL of MgCl2 and standard clone from linearized y-full length transcript.
  • work on snail figures for thesis: snail refinement in AP-twi rm910, and snail dynamics.
  • Confocal on Friday.  To image: snail null + mesodermal genes.  AP-twi rm910.  Also to image: cc12 hb embryos +/- shadow for ML.
  • Start flipping ANB perm fly stocks.
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