cloning y fragment 2nd intron:
Gel extracted bright band amplified from linear full-length yellow.
test gel for probe synthesis, rather variable efficiency.
Probe order: bio-probes (3uL diluted), then dig probes (at 1uL diluted).
hb-full, pnr-exon (A), y-full, sna, ths(A) exon, tup(A) exon, twi, sog.
sog failed, T3 no good? Try to ligate linearized template into pGEM and synthesize with T7 or SP6, in parallel with yellow competition probes.
Forgot ligation reaction from y1 probe.
Repeat in parallel with sog and y2 exon fragment today.