10:00 A — 10:30 P
- Test gel for yellow exon fragment cloning for competition assay
- Test probe synthesis reactions. Results.
- Dot finding code and data (MP01_c collection) to Jacques.
- Gel extract yellow exon fragment clone. High MgCl worked best, cloning from linear full length DNA worked dramatically better than genomic.
- Thesis seminars: Karl (Berger) and Katherine (Marqusee)
- Final probe prep for new in situ probes: precipitate and re-suspend in hybe
- Install NTFS format reader for mac for file conversion things.
- Confocal: warm up no slides, started 5:20 P. Change lens, warm-up again 7:00P-8:30P. Imaging 30 C33 embryos raised at 30C, using 20x objective.
- Ligate sog, y1-exon fragment, y2-exon fragment, negative control, transform comp. cells, plate to grow ON (9:30P).
- Updating Mendeley Library, annotating papers.