Daily Archives: April 12, 2011

updated Q-bio 2011 abstract (submitted)

Posted on April 12, 2011 by admin

Short Abstract — It has been shown that strong gene induction in eukaryotic cells leads to large bursts of gene expression – increasing total mRNA synthesis at the cost of substantially elevating cell-cell variation in expression levels.  We investigate how … Continue reading →

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progress report

Posted on April 12, 2011 by admin

Findings: (1) looping is frequently rate limiting for transcription (see manuscript draft). (2) Bcd activates transcription by affecting promoter priming, not pol II recruitment (unlike most activators from yeast). (3) hb proximal enhancer required for normal levels of total mRNA … Continue reading →

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Imaging bursting?

Posted on April 12, 2011 by admin

Imaging transcriptional bursting directly? .  

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Tuesday 04/12/11

Posted on April 12, 2011 by admin

10:10 A – 7:03 P, 8:10 P – 11:00 P Water Perry’s fly crosses Flip collection cages: 10:45 A Fix embryos: MP09 Hz, MP02 Hz, MP10 Hz; sna[18]/CyoZ (1.5-4.5 hrs) Flip AP-Twi, AP-Twi2 and AP-toll lines Imaging transcriptional bursts (see … Continue reading →

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Monday 04/11/11

Posted on April 12, 2011 by admin

10:00 A – 7:15P, 8:00P – 12:15A revising JF research proposal with dad’s feedback. Confocal too much drift, only 1 image.  needs immediate, 1.5 hr warm up.  extremely frustrating.  1.5 hr imaging of separate slide before finding samples not sufficient. … Continue reading →

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