Daily Archives: April 28, 2011

Probe synthesis

Posted on April 28, 2011 by admin

Making probes.  Reaction setup sketch:     Ordered more bio mix and dig mix.  Enzyme stocks still okay. Attempt new y exon fragment 2 synthesis.

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Thursday 04/28/11

Posted on April 28, 2011 by admin

8:40A – 5:00 P, 10:30 P – 12:00A in vitro transcription reactions (see probe making entry) retry PCR of 2nd exon fragment for y: 1.5 2.5 and 5 uL of MgCl2 and standard clone from linearized y-full length transcript. work … Continue reading →

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