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Daily Archives: April 29, 2011
Friday 04/29/11
10:00 A — 10:30 P Test gel for yellow exon fragment cloning for competition assay Test probe synthesis reactions. Results. Dot finding code and data (MP01_c collection) to Jacques. Gel extract yellow exon fragment clone. High MgCl worked best, cloning … Continue reading
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probe making
cloning y fragment 2nd intron: Gel extracted bright band amplified from linear full-length yellow. test gel for probe synthesis, rather variable efficiency. Probe order: bio-probes (3uL diluted), then dig probes (at 1uL diluted). hb-full, pnr-exon (A), y-full, sna, ths(A) … Continue reading
Posted in probe and plasmid building
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