Daily Archives: April 2, 2011

workshops, 04/02/11

Posted on April 2, 2011 by admin

Pare mRNA counting in Drosophila (McGinnis lab) Recommend acetone permialization. No prot K, need longer hybe. Direct label not as sensitive, much more expensive. More As = better probe (but repeats give background)! Arney et al nut acids 2010. Mounting … Continue reading →

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Techniques Platform, 04/02/11

Posted on April 2, 2011 by admin

Techniques Platform. Perrimon group/ RNAi centers Cell based RNAi screening. Increase in imaging screens (from plate-reader screens). 1-3 million images per std experiment, taken an automatically analyzed. 2-10Tb per screen, currently 35Tb of data. Trying to move to in vivo … Continue reading →

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Platforms 04/02/11

Posted on April 2, 2011 by admin

Regulation of Gene Expression Part I Lehmann lab FACs and RNA-seq to study germline transcription. 7K out 13.8K genes transcribed in germline. Maternal to zygotic transition at stage 8-9 in germ cells. Ovo motif C-GTTA Zeba (dePace Lab) quantitative input … Continue reading →

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