Pare mRNA counting in Drosophila (McGinnis lab)
Recommend acetone permialization.
No prot K, need longer hybe.
Direct label not as sensitive, much more expensive. More As = better probe (but repeats give background)! Arney et al nut acids 2010.
Mounting media recommend prolong. 1 wk freezer curing time
1kb probes with multiple haptens, find both. Shows antibody labelling is good.
deconvolve
Ruth Baker (Oxford)
Rosette formation
2 models, allow point junction boundaries to form vs don’t allow it easily.
Finite element pdes
Scott Barolo
Massively combinatorial.
Need lots of activators to get gene to turn on. Recruit adapters/co-activators.
Similar on outside different on inside? Sushi roll model.
300 bp sequence, all sites matter. Have specialized long range communication elements. Rules about arrangement of binding sites.
Why combinatorial codes? Keeping off as important as keeping on.
Distinguish differential affinity from cooperativity by using single strong site. Looks like a cooperativity thing. Commuted to quantization and (with math models)
Yogi Jaeger
thesis with Walter Gering, ‘he drove me into theorem nice experiments with unfulfilling interpretations.
Kr in mat zygotic hb null gets weaker and moves anterior. Kni is depressor expands in he null, competes with Kr activation. Reverse engineering approach- explain from model on wt data.
Schematic is just binary. How much does the strength?
weak non-symmetric gap interactions necessary to explain dynamics. Clogmia, no posterior he, anterior he less sharp (still does sharpen w/o bcd.
Insulin Tor signaling
via pi3 signaling. Identify novel components using mass-spec.
Ask Spirov about his multiple phenotypes from single clonal mutants.