10:10 A – 7:03 P, 8:10 P – 11:00 P
- Water Perry’s fly crosses
- Flip collection cages: 10:45 A
- Fix embryos: MP09 Hz, MP02 Hz, MP10 Hz; sna[18]/CyoZ (1.5-4.5 hrs)
- Flip AP-Twi, AP-Twi2 and AP-toll lines
- Imaging transcriptional bursts (see post). Using CadN and y lines.
- found bottle of orig MP08s in with MP08 sna[D]s. Fixed contamination issue. 2 new large bottles for collection (all flies individually screened).
- Announce biophysics seminars.
- PCR ths, tup and pnr exonic probes.
- Design and order short yellow probes for competition assay.
- Review and submit Q-bio abstract.