Linearized templates for: inv A, E(Pc) A, tou A, en A (in order).
Gel extracted and purified.
All concentrations ~20 ng/uL.
Synthesis setup: [everything with T7 :), all templates same conc. ].
20x master:
- 100 uL H2O
- 80 uL Tx buffer
- 20 uL DTT
- 40 uL RNAse inhib
- 40 uL T7 pol
- Split 4 ways in 70 uL aliquots. Add 10 uL appropriate hapten (dig, bio, dnp, flu)
- Make 4 15 uL aliquots of each. Add 5 uL of template to each sample.
Order: 1. inv A, 2. E(Pc) A, 3. tou A, 4. en A. rxns labeled by number only. Started at 6:50P.
Confirm synthesis and carb treatment fragmentation.
Loading order: flu 1-4, dnp 1-4, dig 1-4, bio 1-4
