Monthly Archives: January 2016

6:00 pm – 7:00 pm Embryo staining just going for labeling — set heat block to 100C spec new library: label just new library, 3 uL in 20 uL buffer. control embryo, restain Friday’s failed sample with L4E24 P2/S2

9:00 am – 7:15 pm Meetings lab meeting (see notes) embryo staining & imaging stained BX-C locus with Bit 1 (9:00 am) rinsed out stain broke coverslip 1 after it detached from the plastic petri dish before the the nail … Continue reading →

9:40 am – 5:00 pm Goals & progress prep and stain embryos with new L7 library. -> DONE order food for group meeting. -> DONE. pick up suit for interviews. -> DONE. work on equipment lists. -> Extracted parts list … Continue reading →

11:00 am – 6:30 pm embryo smFISH optimization testing quality of methanol stored embryos from yesterday: 6 mo and 2 year embryo batches stained with my in situ protocol I optimized in the Levine lab 6 mo sample directly labelled … Continue reading →

9:40 am – 7:00 pm Reading Recommendations for junior scientists: https://drmaltman.wordpress.com/2014/09/17/10-simple-steps-to-building-a-reputation-as-a-researcher-in-your-early-career/ I don’t have time to make it to the lecture on this today, but some simple suggestions worth bearing in mind from Micha Altman I should probably update my … Continue reading →

10:10 am (back from UCSD interview) Probe synthesis L7 10kb BXC library — amplifying amplifies and saturates about cycle 24 (ran 27 cycles which was probably extra but should still make good probe) purified amplification, ready for T7 reaction don’t … Continue reading →