9:30 am – 9:15 pm
Writing
- working on / revising BW proposal
- two more rounds of comments from Carl
- comments from Jeff.
STORM Analysis
Serious problems introduced by microscope A
- recent bead data sets collected on new version of software are NOT renormalized to maintain full dynamic range and constant contrast! Need to fix this in
imagewriters.py - Dave destroys my conv_bk backup movies, does crazy unexpected stuff with power files and laser levels (blasts my sample with UV). This unanticipated behavior is highly destructive to my sample and data quality.
- Unexpected behavior in dave is due to the entirely different way dave parses shutter files. This needs to be fixed before imaging more.
- Running chromatic bead analysis. Quad view horribly misaligned — require match radius of greater than 30 pixels to match beads.
Literature
- interesting article on transcription vs translation as determinant of protein levels. Also a good example of reusing public data sets.
Cover image
(Good article by Thomas, Hernan and Shawn as well).