Tuesday 08/26/14

10:00 am – 11:30 pm

Writing

  • some revisions to BWF proposal in response to vague comments from XZ.
  • finalize and submit reviewer comments for recent manuscript (review due Wed)

STORM

  • Re-aligning STORM2
  • re-aligned backport optics
  • 561 and 647 beams are no longer aligned prior to backport optics (too much tweaking of the mirrors in front of the lasers…)
  • Tried to balance brightness and flatten field. Upper portion of 647 channel is still a bit dim.
  • Improved alignment of quadview somewhat (less of the field of view is cut off. These shutters and lens controllers are getting pretty sticky…

Chromatin Spot Data collection

  • multiple spots for G10 spots from chrX.
  • Maybe 2 months in culture has been a bit too long for these guys.
  • Started new cells from frozen stock. Seem to be growing alright.

Data analysis

  • Analyzing black domains
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