Tuesday 02/10/15

10:00 am – 7:00 pm, 8:00 pm – 9:45 pm

Project 2

Sequential staining

  • BB reports experiment failed last night. Plan troubleshooting discussion today.
  • apparently a filter issue. staining reported to be sub-par as well.
  • I should probably try these stains myself at some point as a control.

Embryo staining

  • embryos on square cover-glass stained with En look great
  • EN staining is decent given age of antibody
  • took several mosaic regions scans. (probably only saved the EN mosaic and not the WGA. Next time lets save both so we can do the overlay as two separate mosaic files)
  • treated 5 min in Triton, washed with 2x SSC
  • treated 5 min in .1 M HCl, washed with 2x SSC
  • prehybed 10 min in 50% formaimde 2x SSC
  • hybed F02 probe (L3 engrailed locus) with P1 (3 uL of 904) S1 (.6 uL) probe
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