10:15 am – 10:25 pm
Meeting with Guy Nir
- discuss TAD organization
- discuss STORM buffers (see lab protocols)
- discussed STORM analysis – see ZhuangLab Github and tutorials
- discussed converting Nikon format to matlab format.
- Jeff says Nikon has a dax converter available upon request
Computer issues
- Jeff C. fixed firewall issue
- ran windows update (see if the re-introduced firewall blocking server detection of my local computer again)
- to fix, in advanced firewall options, select files and folders
Chromatin
- running export of 2 color data to multi-color panels (for some reason this froze last night)
- analyzing unprocessed black 2 color data. D10-6, D11-7. Looks okay. Didn’t have same-day beads, slight offset I think.
- analyzed D11-6 D12-7 data (these are better)
- analyzing D08 data. This looks good. Got 30 cells done. Should compare to rest of data to see how it looks.
Coding
- fixed bug in Translate image
- updated substantially PlotBlobExample3D and Plot2Color3Dchromatin in Chromatin\Code\Functions.
- we really want this built into the Cropper.
- added new options to adjust contrast of conventional images being used for auto-correlation image alignment (this works much better).