9:00 am – 10:50 pm
Computer maintenance
- finish install of updates. Shut-down Morgan
- install power adapter for USB 3.0 cards bridge to DVD drives
- investigate motherboard for ID# (Dell special, no additional info) and RAID card (none evident)
- restart computer
- Morgan still not recognizing added disk drives.
- formatted one of the disks on Monet and remounted in Morgan. Still not recognized
- rebooted Morgan. Drive still not recognized. 🙁
- swapping existing F with new drive (turned off).
- mounted wrong in bracket. Needs to be in SAS, not SATAmu position.
- recognizes top rack of drives. Still not recognizing bottom rack, despite the fact these clearly do plug into the motherboard.
Further investigation of the issues
- http://en.community.dell.com/support-forums/servers/f/906/t/19568567
- try customer service chat tomorrow morning
Embryo work
- polyD lysine coated second rack of coverslips (others are 5x chromium gelatin)
- polyD lysine coverslips easily ID’d from salt residue
- sectioned embryos, used all coverslips from both rack
- cut until validated that sections contain embryos.
- mostly cut at at 2 um, -62C blade and mount, -55C chamber. Sections coming very nicely off blade today, limited coiling.
- ordered new loops for ultra-cryo section collection
- froze along with the pin
MERFISH
- Processing probes in 15-04-19_Probes_18mers_t66Cx76Cs99C
- 18 – 22mers.
- 186 genes in my list have non-unique common names / gene symbols
- 5,015 have FPKM > 0.1 (max over cell lines) and more than 100 probes.
- Maybe 76C is too strict for off target homology cut.
Human transcriptome rebuild
- Currently building annotation off human chromosome fasta files.
- sent new transcriptome fasta file to Jeff for all protein coding genes