Thursday 04/30/15

Posted on April 30, 2015 by admin

9:00 am – 7:30 pm, 9:00 pm – 11:30 pm

Thesis defense

  • finalizing hat for Hao 9am-10am
  • Hao’s Thesis defense 10am-11:15 am
  • more finalizing hat 11:15-11:45

Discussions 11:45-1:00pm

  • discussion of AO with HB and BB
  • discussion of BB’s sequential stain data — look’s awesome!

Presentation Prep

  • working on slides for CSHL meeting
  • working on slides for Ph-polymerization project summary for tomorrow lab meeting

MERFISH

Developing New Pipeline for Multicolor data 7:00pm – 11:20 pm

  • attempting pre-warp
  • using brightfield bead movies and CorrAlign
  • adjusted CorrAlign to dilate images before computing correlation
    • this is computationally expensive. 5x is quite slow. 3x is okay.
    • this does provide sub-pixel improvement
  • TranslateImage however is pixel based, doesn’t provide sub-pixel translation.
    • could make sub-pixel version — just dilate, then translate.

Questions for team

  • why 3 color 8 hybes for L7 (only 16 bits not 24…)
  • there are clearly focus lock issues still…
  • why is 10 uM scale of primer $400!! (it would be cheaper to order 10x 1uM scale, ~$300)

Ph Project

  • reply to NJ about abstract
  • to do tomorrow: rewrite model discussion section
  • re-arrange the rest of the Fig 5 panels
This entry was posted in Summaries. Bookmark the permalink.