Wednesday 07/08/15

9:30 am – 9:15 pm

MERFISH

  • working on aligning data
  • current pentanotch no quadview approach requires different approach to beads
  • try multilabeled beads (homemade tetraspec with 750 dyes)
  • try using labeled cells

Chromatin meeting with XZ

  • priority on PcG knockdown
  • use immunostain of protein to quantify protein efficiency KD
  • try to increase KD efficiency of individual KDs.
  • keep focus on multi component KD for imaging work

Apps and Conferences

  • finalize DF files, submit to Matt
  • send signature form for internal signatures
  • working on slides for SDB meeting
  • getting a few things for ML party at SDB

To order

  • secondary sequences fused to common sequence
  • more RNAi primers
  • more PCR primers
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