11:00 am – 7:30 pm, 9:30 pm – 10:45 pm
(9:00 am – 11:00 am volleyball practice)
Chromatin
Cell
- plated Kc167 cells (in SFX) on glass
- my more recent flask with the short Trypsin treatment is doing well.
- the original passage with the longer Trypsin exposure is not looking so good.
- tried removing Psc- cells by blowing (without Trypsin). works a little bit, not at all like with Trypsin.
- added a little scraping an moved cells to new vial
- tried imaging cells live labeled with Hoechst – doesn’t bleach much or blink
- tried imaging cells live labeled with Draq5 – doesn’t bleach at all (or switch to dark state to blink)
- tried both culture media and STORM BME imaging buffer.
- Invitrogen has a number of other cell-permiable dyes: https://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/probes-for-organelles/nuclear-and-chromosome-counterstaining-and-nissl-stains.html#head1
Cell cycle markers
Culturing Psc- cells
- tried passage by scrapping — disaester, cells die
- tried trypsin first spin looks good. No cells came down in PBS spin (sparse cells). Don’t look good on plates. I think I killed these.