9:45 am – 11:15 pm
Batch data analysis of confocal live imaging of fixation effects
Matched feature aligned images:
Positive Control:
A few more examples of positive control
Signed up for Elyra training
MERFISH
- analyzing L12 data
- see notebook post
STORM
- imaging session start delayed to 6:30 pm (from 5:00 pm).
- attempting STORM imaging of L2F04 Ph-KD on STORM2
- focus lock doesn’t work (defocuses)
- 561 laser intensity very low (turned out to be the half-wave-plate polarized the beam in the wrong direction)
- switched to STORM3
- quadview is badly out of alignment (apparently due to mis-treatment of the unit?)
- beam behaves as if there’s a fun-mirror/lens in the path causing very weird distortions to pointspread function as spots move in and out of focus.
- aborted attempt to image tonight (~11:15 pm).